Walnut Processed by Subcritical Extraction: An Innovative Product with High Nutritional Value

Walnuts are widely known for their health benefits, rich composition, and versatility in use. Modern processing methods allow for maximum preservation of their biological value, with subcritical extraction
being one of the most promising approaches.

Subcritical Extraction Method and Its Advantages

Subcritical extraction is an advanced technology that utilizes mild solvents at low temperatures (typically below 50°C) and moderate pressure. Unlike traditional methods (organic solvent extraction or mechanical pressing), this approach allows for:
• Preservation of proteins and beneficial fatty acids without thermal or chemical degradation.
• Oil extraction and removal of excess fat from the walnut, increasing protein concentration and improving digestibility (Best Extraction Machine, 2024).
• Reduction of anti-nutritional factors, such as enzyme inhibitors, thereby enhancing nutritional value (Scientific Research, 2024). 

Composition and Nutritional Value of the Processed Product

After subcritical extraction, walnuts retain all essential macro- and micronutrients while acquiring enhanced properties:
Component /  Content
Proteins  / 50-60% (after defatting)
Omega-3 and Omega-6  / High concentration
Vitamins / E, B-group (B1, B2, B6, B9)
Antioxidants  / Flavonoids, polyphenols
Phospholipids / Lecithin, phosphatidylcholine, phosphatidylserine

Walnut Peptides and Their Health Benefits

Peptides derived from walnut proteins exhibit various biological activities, providing multiple health benefits:
• Antioxidant activity: Peptides neutralize free radicals, reducing oxidative stress and preventing cell damage (PubMed, 2024). This is crucial for aging prevention and protection against chronic diseases.
• Antihypertensive effects: Certain peptides inhibit angiotensin-converting enzyme (ACE), helping to lower blood pressure (ScienceDirect, 2024). This makes them beneficial for individuals with
hypertension and cardiovascular diseases.
• Anti-inflammatory properties: Peptides suppress inflammatory cytokines, helping to reduce inflammation in the body and alleviate symptoms of chronic diseases, including arthritis and autoimmune disorders.
• Anti-fatigue effects: Walnut oligopeptides enhance endurance, reduce fatigue, and accelerate postexercise recovery (Alfa Chemistry, 2024). This makes the product highly sought after by athletes.
• Neuroprotective properties: Certain peptides can improve cognitive function, enhance memory, and reduce stress levels. They support nervous system health, helping combat age-related brain decline and concentration issues (ScienceDirect, 2023).
• Immune system support: Protein peptides activate the body's defense mechanisms, enhance immunoglobulin synthesis, and increase resistance to infections.
• Blood sugar regulation: Walnuts improve insulin sensitivity and stabilize blood glucose levels, making them beneficial for individuals with diabetes and metabolic disorders. 

Clinical Research Findings

Cholesterol reduction: A 2023 study showed that patients consuming walnuts processed by subcritical extraction experienced a significant decrease in cholesterol levels and improved vascular tone (PubMed, 2023).
• Cognitive function improvement: Another study found that walnut protein peptides enhance focus and reduce stress levels in students (ScienceDirect, 2023).
• Enhanced physical endurance: Experimental data confirm that walnut peptides can significantly improve athletic performance and speed up recovery after training. 

Amino Acid Profile

Walnuts are a valuable source of essential amino acids, particularly arginine, which is crucial for cardiovascular health.
Amino Acid Content (per 100g of protein)
Arginine - 21.5g
Glutamic acid - 18.2g
Aspartic acid - 9.1g
Leucine - 6.8g
Lysine - 4.2g
Valine - 4.6g
Isoleucine - 3.9g
Tryptophan - 1.1g

Who Needs This Product? Target Groups

1. Athletes and active individuals – High protein and amino acid content aids muscle recovery after workouts.
2. People with high blood pressure – Walnut peptides help lower blood pressure and improve vascular health.
3. Elderly individuals – High concentrations of antioxidants and phospholipids support cognitive function and slow down aging processes.
4. Students and office workers – Phosphatidylserine and lecithin enhance memory and concentration.
5. Individuals with metabolic disorders – Walnuts aid in regulating blood sugar and lipid levels. 

Conclusion

Walnuts processed by subcritical extraction are a unique, highly nutritious product with broad applications in
the food industry, sports nutrition, and functional foods. They combine natural taste, enhanced bioavailability,
and extended shelf life, making them a valuable ingredient in the health food industry